The company recently shifted production of the GMC Terrain to Mexico, eliminating 600 jobs at the Canadian plant.
Union workers at General Motors' CAMI Assembly plant in Ingersoll, Ontario, have gone on strike after failing to negotiate a labor agreement.
"Your master bargaining committee has not been successful in securing a tentative agreement with the company," Unifor wrote in a statement to around 2,800 workers at the plant, as quoted by CBC.
Located not far from London, Ontario, the factory was established three decades ago via a joint venture with Suzuki. The plant is currently focused on the Chevrolet Equinox, helping keep up with a 17-percent increase in demand for GM's most popular crossover in the US.
Approximately 600 workers were recently laid off when GM ceased production of the GMC Terrain at CAMI Assembly, paving the way for a switch to Mexico.
"While General Motors of Canada and our Unifor partners have made very positive progress on several issues over the past weeks, the Company is disappointed that we were not able to complete a new agreement," the company said in a statement. "We encourage Unifor to resume negotiations and to continue working together to secure a competitive agreement."